The bioassay data displayed in this panel includes modeled AC50 values from multi-concentration data only. Single concentration data may also be available for some assay technologies. Single concentration data are included in the list of ‘tested’ assays endpoints if multi-concentration data are unavailable. Raw, normalized, and interpreted single concentration data from level 0 to level 2 are fully available from the freely downloadable MySQL database, invitrodb. Level 0 is the raw data from the vendor, level 1 takes the raw values and normalizes them to either the percentage of a control or to fold-change from baseline, and level 2 processing defines the baseline median absolute deviation (bmad), collapses any replicates by sample ID, and determines the activity. The scaled response shown on the graph below is calculated by dividing the response values by the activity cutoff thereby enabling response comparisons across assay endpoints.
The ToxCast: Summary sub-tab renders a summary plot of assay results for common assay targets. Hovering the mouse over points on the plot provides more information, some points include a link to a report accessible by clicking. A drop-down menu at the top of the plot switches between linear and log scale. The cytotoxicity median and lower bound estimates are based on as many as 88 assay endpoints in invitrodb including multiple cell lines and technologies and represent an estimated concentration for general cytotoxicity across assays. These values may provide useful context for interpreting ToxCast results.
Below the plot is a table of summary data. The table loads with a filter to show only Active responses, Inactive responses can be added by clicking on the filter icon in the Hit Call column and checking “Select All”. Hovering on the underlined assay names in the Name column shows a short description of the assay. The document icons in the Details column link to more detailed, downloadable, descriptions of the assays.
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